首页> 外文OA文献 >Cellular tumorigenicity in nude mice. Test of associations among loss of cell-surface fibronectin, anchorage independence, and tumor-forming ability
【2h】

Cellular tumorigenicity in nude mice. Test of associations among loss of cell-surface fibronectin, anchorage independence, and tumor-forming ability

机译:裸鼠的细胞致瘤性。测试细胞表面纤连蛋白的丧失,锚定独立性和肿瘤形成能力之间的关联

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务,本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文,但由于OA文献来源多样且变更频繁,仍可能出现获取不到、文献不完整或与标题不符等情况,如果获取不到我们将提供退款服务。请知悉。
获取外文期刊封面目录资料

摘要

Fibronectin (FN; also called large external transformation-sensitive [LETS] protein or cell-surface protein [CSP]) is a large cell-surface glycoprotein that is frequently observed to be either absent or greatly reduced on the surfaces of malignant cells grown in vitro. Because FN may be a useful molecular marker of cellular malignancy, we have carried out an extensive screening to test the specific association among the degree of expression of FN, anchorage-independent growth, and tumorigenicity in the athymic nude mouse. A variety of diploid cell strains and established cell lines were tested for the expression of surface FN by indirect immunofluorescence using rabbit antisera against human cold insoluble globulin, rodent plasma FN, or chicken cell- surface FN. Concomitantly, the cells were assayed for tumor formation in nude mice and for the ability to form colonies in methylcellulose. Tumorigenic cells often showed very low surface fluorescence, confirming earlier reports. However, many highly tumorigenic fibroblast lines from several species stained strongly with all three antisera. In contrast, the anchorage-independent phenotype was nearly always associated with tumorigenicity in approximately 35 cell lines examined in this study. In another series of experiments, FN-positive but anchorage-independent cells were grown as tumors in nude mice and then reintroduced into culture. In five of the six tumor-derived cell lines, cell-surface FN was not significantly reduced; one such cell line showed very little surface FN. Our data thus indicate that the loss of cell-surface FN is not a necessary step in the process of malignant transformation and that the growth of FN-positive cells as tumors does not require a prior selection in vivo for FN-negative subpopulations.
机译:纤连蛋白(FN;也称为大的外部转化敏感[LETS]蛋白或细胞表面蛋白[CSP])是一种大的细胞表面糖蛋白,通常观察到在其中生长的恶性细胞表面上不存在或大大减少了体外。由于FN可能是细胞恶性肿瘤的有用分子标记,因此我们进行了广泛的筛选,以测试FN的表达程度,不依赖贴壁的生长和无胸腺裸鼠的致瘤性之间的特定关联。使用兔抗人冷不溶性球蛋白,鼠血浆FN或鸡细胞表面FN的抗血清,通过间接免疫荧光测试了各种二倍体细胞株和已建立的细胞系表面FN的表达。伴随地,分析了细胞在裸鼠中的肿瘤形成以及在甲基纤维素中形成集落的能力。致瘤细胞通常显示出非常低的表面荧光,这证实了较早的报道。但是,来自多个物种的许多高度致瘤性成纤维细胞系均被这三种抗血清强烈染色。相反,在这项研究中检测的约35个细胞系中,与锚定无关的表型几乎总是与致瘤性相关。在另一系列实验中,FN阳性但不依赖贴壁的细胞在裸鼠中作为肿瘤生长,然后重新引入培养物中。在六个肿瘤来源的细胞系中的五个中,细胞表面FN并未显着降低;一种这样的细胞系显示出非常小的表面FN。因此,我们的数据表明,细胞表面FN的丧失不是恶性转化过程中的必要步骤,并且由于肿瘤的FN阳性细胞的生长不需要在体内进行FN阴性亚群的预先选择。

著录项

  • 作者

  • 作者单位
  • 年度 1979
  • 总页数
  • 原文格式 PDF
  • 正文语种 {"code":"en","name":"English","id":9}
  • 中图分类

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号